Plant amino acid metabolism

Amino acids are involved in several physiological processes in plants apart from constituting proteins. Plant amino acid metabolism strongly reacts to challenges caused by both, biotic as well as abiotic stress conditions and provides a diverse set of signaling and defense compounds. Our group aims to identify the presently unknown reaction steps and pathways involved in amino acid catabolism. Using drought stress and germination as model conditions for increased protein turnover we study amino acid metabolism in Arabidopsis thaliana and Lupinus albus on a quantivitve level. We are also interested in the biochemical mechanisms of amino acid sensing and signaling.

Original Publications

  • Heinemann, B. and Hildebrandt, T.M. (2021): The role of amino acid metabolism in signaling and metabolic adaptation to stress-induced energy deficiency in plants.J Exp Bot 72, 4634-4645.
    DOI: 10.1093/jxb/erab182
  • Heinemann, B., Künzler, P., Braun, H.P. and Hildebrandt, T.M. (2021): Estimating the number of protein molecules in a plant cell: a quantitative perspective on proteostasis and amino acid homeostasis during drought.Plant Physiol 185, 385–404.
    DOI: 10.1093/plphys/kiaa050
  • Moseler A, Kruse I, Maclean AE, Pedroletti L, Franceschetti M, Wagner S, Wehler R, Fischer-Schrader K, Poschet G, Wirtz M, Dörmann P, Hildebrandt TM, Hell R, Schwarzländer M, Balk J and Meyer A (2021): The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria.Plant Physiol 186, 1507-1525.
    DOI: 0.1093/plphys/kiab172
  • Fuchs, P., Rugen, N., Carrie, C., Elsässer, M., Finkemeier, I., Giese, J., Hildebrandt, T., Kühn, K., Maurino, V., Ruberti, C., Schallenberg-Rüdinger, M., Steinbeck, J., Braun, H.P., Eubel, H., Meyer, E.H., Müller-Schüssele, S. and Schwarzländer, M. (2020): Single organelle function and organization as estimated from Arabidopsis mitochondrial proteomics.Plant J. 101, 420–441.
    DOI: 10.1111/tpj.14534
  • Batista-Silva W, Heinemann B, Rugen N, Nunes-Nesi A, Araújo WL, Braun HP, Hildebrandt TM. (2019): The role of amino acid metabolism during abiotic stress release.Plant Cell Environ. 2019 May;42(5):1630-1644.
    DOI: 10.1111/pce.13518
  • Farhat, N., Hichri, S., Hildebrandt, T.M., Debez, A. and Braun, H.P. (2019) (2019): Composition and stability of the Oxidative Phosphorylation system in the halophile plant Cakile maritima.Frontiers in Plant Sciences 10, 1010
    DOI: 10.3389/fpls.2019.01010
  • Belghith I, Senkler J, Hildebrandt TM, Abdelly C, Braun HP & Debez A (2018): Comparative analysis of salt-induced changes in the root proteome of two accessions of the halophyte Cakile maritima.Plant Physiol Biochem 130, 20-29.
    DOI: 10.1016/j.plaphy.2018.06.029
  • Brandt S, Fachinger S, Tohge T, Fernie AR, Braun HP & Hildebrandt TM (2018): Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves.PloS one 13 (8), e0202153.
  • Hildebrandt TM (2018): Synthesis versus degradation: directions of amino acid metabolism during Arabidopsis abiotic stress response.Plant Mol Biol 98, 121-135.
    DOI: 10.1007/s11103-018-0767-0
  • Lorenz C, Brandt S, Borisjuk L, Rolletschek H, Heinzel N, Tohge T, Fernie AR, Braun HP & Hildebrandt TM (2018): The role of persulfide metabolism during Arabidopsis seed development under light and dark conditions. Front Plant Sci, Research Topic “Hydrogen Sulfide Metabolism and Signalling in Plants” 9, 1381.
    DOI: 10.3389/fpls.2018.01381
  • Cavalcanti J.H., Quinhones, C.G., Schertl, P., Brito, D.S., Hildebrandt, T., Nunes-Nesi, A., Braun, H.P. and Araújo, WL (2017): The differential impact of amino acids on OXPHOS system activity following carbohydrate starvation in Arabidopsis cell suspensions.Physiologia Plantarum 161, 451–467.
    DOI: 10.1111/ppl.12612
  • Luna‐Sánchez M, Hidalgo‐Gutiérrez A, Hildebrandt TM, Chaves‐Serrano J, Barriocanal‐Casado E, Santos‐Fandila A, Romero M, Sayed RKA, Duarte J, Prokisch H, Schuelke M, Distelmaier F, Escames G, Acuña‐Castroviejo D & López LC (2017): CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndromeEMBO Mol Med 9: 78-95
    DOI: 10.15252/emmm.201606345
  • Senkler, J., Senkler, M., Eubel, H., Hildebrandt, T., Lengwenus, C., Schertl, P., Schwarzländer, M., Wagner, S., Wittig, I., and Braun, H.P. (2017): The mitochondrial complexome of Arabidopsis thaliana.Plant J., 89, 1079-1092
    DOI: 10.1111/tpj.13448
  • Höfler, S., Lorenz, C., Busch, T., Brinkkötter, M., Tohge, T., Fernie, A., Braun, H.P. and Hil-debrandt, T. (2016): Dealing with the sulfur part of cysteine: four enzymatic steps degrade l-cysteine to pyruvate and thiosulfate in Arabidopsis mitochondria.Physiologia Plantarum 157, 352-366 More info
  • Birke, H., Hildebrandt, T.M., Wirtz, M., and Hell, R. (2015): Sulfide detoxification in plant mitochondria.Methods Enzymol 555: 271-86. More info
  • Hildebrandt, T., Nunes Nesi, A., Araujo, W.L. and Braun, H.P. (2015): Amino acid catabolism in plants.Mol. Plant 8, 1563-1579. More info
  • Schwender, J., Hebbelmann, I. Heinzel, N., Hildebrandt, T.M., Rogers, A., Naik, D., Klapperstück, M., Braun, H.P., Schreiber, F., Denolf, P., Borisjuk, L. and Rolletschek, H. (2015): Quantitative multilevel analysis of central metabolism in developing oilseeds of Brassica napus during in vitro culture.Plant Physiol. 168, 828-348. More info
  • Krüßel, L., Junemann, J., Wirtz, M., Birke, H., Thornton, JD, Browning, L.W., Poschet, G., Hell, R., Balk, J., Braun, H.P. and Hildebrandt, T.M. (2014): The mitochondrial sulfur dioxygenase ETHE1 is required for amino acid catabolism during carbohydrate starvation and embryo development in Arabidopsis thaliana.Plant Physiol. 165, 92-104 More info
  • Hildebrandt, H., Viscomi, C., Di Meo, I, Zeviani, M. and Braun, H.P. (2013): Proteome adaptations in Ethe1 deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications.Biosci. Rep. 33, art:e00052, doi 10.1042/BSR20130051 More info
  • Welchen, E., Hildebrandt, T., Lewejohann, D., Gonzalez, D. and Braun, H.P. (2012): Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes I and III.Biochim Biophys Acta. 2012 Apr 19;1817(7):990-1001 More info
  • Farese, S., Stauffer, E., Kalicki, R., Hildebrandt, T., Frey, B.M., Frey, F.J., Uehlinger, D.E. and Pasch, A. (2011): Sodium thiosulfate pharmacokinetics in hemodialysis patients and healthy volunteers.Clin J Am Soc Nephrol 6, 1447-1455.
  • Hildebrandt, T. (2011): Biochem. Biophy. Acta (Bioenergetics) 1807, 1206-1213Biochem. Biophy. Acta (Bioenergetics) 1807, 1206-1213
  • Hildebrandt, T.M. (2011): Modulation of sulfide oxidation and toxicity in rat mitochondria by dehydroascorbic acid.Biochim Biophys Acta – Bioenergetics 1807(9):1206-13 More info
  • Jonietz, C., Forner, J., Hildebrandt, T. and Binder, S. (2011): RNA PROCESSING FACTOR3 Is Crucial for the Accumulation of Mature ccmC Transcripts in Mitochondria of Arabidopsis Accession Columbia.Plant Physiol. 157, 1430-1439.
  • Viscomi, C., Burlina, A.B., Dweikat, I., Savoiardo, M., Lamperti, C., Hildebrandt, T., Tiranti, V. and Zeviani, M. (2010): Combined treatment with oral metronidazole and N-acetylcysteine is effective in ethylmalonic encephalopathy.Nat Med 16, 869-871.
  • Krueger, S., Niehl, A., Martin, M.C.L., Steinhauser, D., Donath, A., Hildebrandt, T., Romero, L.C., Hoefgen, R., Gotor, C. and Hesse, H. (2009): Analysis of Cytosolic and Plastidic Serine Acetyltransferase Mutants and Subcellular Metabolite Distributions Suggests Interplay of the Cellular Compartments for Cysteine Biosynthesis in Arabidopsis.Plant Cell Environ 32, 349-367.
  • Tiranti, V., Viscomi, C., Hildebrandt, T.M., DiMeo, I., Mineri, R., Tiveron, C., Levitt, M., Prelle, A., Rimoldi, M. and Zeviani, M. (2009): Loss of ETHE1, a mitochondrial dioxygenase, causes fatal sulfide toxicity in Ethylmalonic Encephalopathy.Nat Med 15, 200-205.
  • Hildebrandt, T.M. and Grieshaber, M.K. (2008): Redox regulation of mitochondrial sulfide oxidation in the lugworm, Arenicola marina.J Exp Biol 211, 2617-2623.
  • Hildebrandt, T.M. and Grieshaber, M.K. (2008): Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria.FEBS J 275, 3352-3361.

Other Publications

  • Hildebrandt, T. and Grieshaber, M.K. (2009): Tödlich und doch lebensnotwendig: Die vielen Seiten des Sulfids.Biologie in unserer Zeit 39, 328-334