The aim of LACoP is the production of food plants with a reduced content of the allergenic storage proteins 2S albumin Bra j 1 (mustard) and 7S globulin Ara h 1 (peanut). Only the allergenic epitopes are removed from the corresponding genes by genome editing.
The advantage of CRISPR-Cas9 based genome editing is that several mutations can be introduced simultaneously at different sites in the genome. This is important because many allergenic proteins have a polygenic background.
In this BMBF-funded cooperation project, the task of our research group is to produce allergenic proteins in different systems (E. coli, Wolffia australiana, microalgae) and make them available to our partners as purified proteins.
