Original Publications

  • Annett Erkes, Stefanie Mücke, Maik Reschke, Jens Boch, Jan Grau (2019): PrediTALE: A novel model learned from quantitative data allows for new perspectives on TALE targetingbioRxiv 522458
  • Stefanie Mücke, Maik Reschke, Annett Erkes, Claudia-Alice Schwietzer, Sebastian Becker, Jana Streubel, Richard D. Morgan, Geoffrey G. Wilson, Jan Grau and Jens Boch (2019): Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEsFront. Plant Sci., 25 February 2019
  • Ordon, J., Gantner, J., Kemna, J., Schwalgun, L., Reschke, M., Streubel, J., Boch, J., and Stuttmann, J. (2017): Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkitPlant J. 89, 155-168 More Info
    ISBN: 10.1111/tpj.13319
  • Streubel, J., Baum, H., Grau, J., Stuttman, J., and Boch, J. (2017): Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. PLoS ONE 12, e0173580
  • Volohonsky, G., Hopp, A.-K., Saenger, M., Soichot, J., Scholze, H., Boch, J., Blandin, S.A., Marois, E. (2017): Transgenic expression of the anti-parasitic factor TEP1 in the malaria mosquito Anopheles gambiaePLoS Pathog. 13, e1006113 More Info
    DOI: 10.1371/journal.ppat.1006113
  • Becker, S., Boch, J. (2016): Single-molecule biophysics: TALEs spin along, but not aroundNat. Chem. Biol. 12, 766-768 More Info
  • Grau, J., Reschke, M., Erkes, A., Streubel, J., Morgan, R.D., Wilson, G.G., Koebnik, R. and Boch, J. (2016): AnnoTALE: bioinformatics tools for identification, annotation, and nomenclature of TALEs from Xanthomonas genomic sequencesSci. Rep. 6, 21077 More Info
    DOI: 10.1038/srep21077
  • Hutin, M., Césari, S., Chalvon, V., Michel, C., Tran, T.T., Boch, J., Koebnik, R., Szurek, B., and Kroj, T. (2016): Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseasesPlant J. 88, 43-55 More Info
  • Linda Kirchner, Alison Wirshing, Lutfiye Kurt, Thomas Reinard, James Glick, Erin J. Cram, Hans-Jörg Jacobsen, Carolyn W.T. Lee-Parsons (2016): Identification, characterization, and expression of diacylgylcerol acyltransferase type-1 from Chlorella vulgarisAlgal Research, Volume 13, January 2016, Pages 167–181
    DOI: 10.1016/j.algal.2015.10.017
  • Geissler, R., Hauber, I., Funk, N., Richter, A., Behrens, M., Renner, I., Chemnitz, J., Hofmann-Sieber, H., Baum, H., van Lunzen, J., Boch, J., Hauber, J., and Behrens, S.E. (2015): Patient-adapted, specific activation of HIV-1 by customized TAL effectors (TALEs), a proof of principle study.Virology 488:248-254. More Info
  • Vierthaler, Reinard, Glasmacher, Hofmann (2015): The use of an ice-binding-protein out of the snowflea Hypogastrura harveyi as a cryoprotectant in the cryopreservation of mesenchymal stem cells Current Directions in Biomedical Engineering 2015 [1]
  • Boch, J., Bonas, U., and Lahaye, T. (2014): TAL effectors - pathogen strategies and plant resistance engineeringNew Phytol. 204, 823-832 More Info
    DOI: 10.1111/nph.13015
  • Richter, A., Streubel, J., Blücher, C., Szurek, B., Reschke, M., Grau, J., and Boch, J. (2014): A TAL effector repeat architecture for frameshift binding.Nat. Commun. 5, 3447. More Info
  • Arrieta-Ortiz, M.L., Rodriguez-R., L.M., Perez-Quintero, A.L., Poulin, L., Diaz, A.C., Rojas, N.A., Trujillo, C., Restrepo Benavides, M., Bart, R., Boch, J., Boureau, T., Darrasse, A., David, P., Duge de Bernonville, T., Fontanilla, P., Gagnevin, L., Guerin, F., Jacques, M.-A., Lauber, E., Lefeuvre, P., Medina, C., Medina, E., Montenegro, N., Munoz Bodnar, A., Noel L.D., Ortiz Quinones, J.F., Osorio, D., Pardo, C., Patil, P.B., Poussier, S., Pruvost, O., Robene-Soustrade, I., Ryan, R.P., Tabima, J., Urrego Morales, O.G., Verniere, C., Carrere, S., Verdier, V., Szurek, B., Restrepo, S., Lopez, C., Koebnik, R., Bernal, A. (2013): Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151 PLoS ONE 8, e79704 More Info
    DOI: 10.1371/journal.pone.0079704
  • Grau, J., Boch, J., and Posch, S. (2013): TALENoffer: genome-wide TALEN off-target prediction. Bioinformatics 29, 2931-2932. More Info
  • Grau, J., Wolf, A., Reschke, M., Bonas, U., Posch, S., and Boch, J. (2013): Computational predictions provide insights into the biology of TAL effector target sites. PLoS Comp. Biol. 9, e1002962. More Info
  • Richter, A. and Boch, J. (2013): Designer TALEs team up for highly efficient gene induction. Nat. Methods 10, 207-208. More Info
  • Streubel, J., Pesce, C., Hutin, M., Koebnik, R., Boch, J., and Szurek, B. (2013): Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae. New Phytol. 200, 808-819. More Info
  • Streubel, J., Richter, A., Reschke, M., and Boch, J. (2013): TALEs - Proteine mit programmierbarer DNA-Bindespezifität. BIOspektrum 19, 370-372. More Info
  • Nau-Wagner, G., Opper, D., Rolbetzki, A., Boch, J., Kempf, B., Hoffmann, T., and Bremer, E. (2012): Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor. J. Bacteriol. 194, 2703-2714. More Info
  • Streubel, J., Blücher, C., Landgraf, A., and Boch, J. (2012): TAL effector RVD specificities and efficiencies. Nat. Biotechnol. 30, 593-595. More Info
  • Boch, J. (2011): TALEs of genome targeting.Nat. Biotechnol. 29, 135-136. More Info
  • Geißler, R., Scholze, H., Hahn, S., Streubel, J., Bonas, U., Behrens, S.-E., and Boch, J. (2011): Transcriptional activators of human genes with programmable DNA-specificity. PLoS ONE 6, e19509. More Info
  • Scholze, H. and Boch, J. (2011): TAL effectors are remote controls for gene activation.Curr. Opin. Microbiol. 14, 47-53. More Info
  • Yu, Y., Streubel, J., Balzergue, S., Champion, A., Boch, J., Koebnik, R., Feng, J.-X., Verdier, V., and Szurek, B. (2011): Colonization of rice leaf blades by an African strain of Xanthomonas oryzae pv. oryzae depends on a new TAL effector which induces the rice nodulin-3 Os11N3 gene. Mol. Plant-Microbe Interact. 24, 1102-1113. More Info
  • Boch, J. and Bonas, U. (2010): Xanthomonas AvrBs3 family-type III effectors: discovery and function. Annu. Rev. Phytopathol. 48, 419-436. More Info
  • Ferro N., Bredow T., Jacobsen H.-J., Reinard T. (2010): Route to Novel Auxin: Auxin Chemical Space toward Biological Correlation Carriers.Chem Rev. 2010 Aug 11;110(8):4690-708
    DOI: 10.1021/cr800229s
  • Morbitzer, R., Römer, P., Boch, J., and Lahaye, T. (2010): Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors.Proc. Natl. Acad. Sci. USA 107, 21617-21622. More Info
  • Römer, P., Recht, S., Strauß, T., Elsaesser, J., Schornack, S., Boch, J., Wang, S., and Lahaye, T. (2010): Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. New Phytol. 187, 1048-1057. More Info
  • Scholze, H. and Boch, J. (2010): TAL effector-DNA specificity. Virulence 1, 428-432. More Info
  • Boch, J., Scholze, H., Schornack S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., and Bonas, U. (2009): Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326, 1509-1512; published online 29 October 2009 (10.1126/science.1178811). More Info
  • Ferro N, Bultinck, P, Gallegos, A, Jacobsen, H-J, Carbo-Dorca, R, Reinard, T. (2007): Unrevealed structural requirements for auxin-like molecules by theoretical and experimental evidences.Phytochemistry, 2007, 68 (2) 237-250.
    DOI: 10.1016/j.phytochem.2006.10.006
  • de Torres, M., Mansfield, J., Grabov, N., Brown, I.R., Ammouneh, H., Tsiamis, G., Forsyth, A., Robatzek, S., Grant, M., and Boch, J. (2006): Pseudomonas syringae effector AvrPtoB suppresses basal defence in Arabidopsis. Plant J. 47, 368-382. More Info
  • Ferro N, Gallegos, A, Bultinck, P, Jacobsen, H-J, Carbo-Dorca, R, Reinard, T. (2006): Coulomb and Overlap Self - Similarities: A comparative selectivity analysis of structure - function relationships for auxin - like molecules.J. Chem. Inf. Modeling (JCICS) 2006, 46(4):1751-1762
    DOI: 10.1021/ci050491c
  • Ferro N, Tacoronte JE, Bultinck P, Reinard T, Montero LA. (2006): Structure–activity analysis on ecdysteroids: A structural and quantum chemical approach based on two biological systems.Journal of Molecular Structure: THEOCHEM 2006; 758, 2-3, 263-274
    DOI: 10.1016/j.theochem.2005.10.027
  • Krause, A., Ramakumar, A., Bartels, D., Battistoni, F., Bekel, T., Boch, J., Böhm, M., Friedrich, F., Hurek, T., Krause, L., Linke, B., McHardy, A.C., Sarkar, A., Schneiker, S., Syed, A.A., Thauer, R., Vorhölter, F.J., Weidner, S., Pühler, A., Reinhold-Hurek, B., Kaiser, O., and Goesmann, A. (2006): Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72.Nat. Biotechnol. 24, 1385-1391. More Info
  • Landgraf, A., Weingart, H., Tsiamis, H., and Boch, J. (2006): Different versions of Pseudomonas syringae pv. tomato DC3000 exist due to the activity of an effector transposon. Mol. Plant Pathol. 7, 355-364. More Info
  • Kabisch, U., Landgraf, A., Krause, J., Bonas, U., and Boch, J. (2005): Type III secretion chaperones ShcS1 and ShcO1 from Pseudomonas syringae pv. tomato DC3000 bind more than one effector.Microbiology 151, 269-280.
  • Kay, S., Boch, J., and Bonas, U. (2005): Characterization of AvrBs3-like effectors from a Brassaceae pathogen reveals virulence and avirulence activities and a protein with a novel repeat architecture.Mol. Plant-Microbe Interact. 18, 838-848. More Info
  • Lauer B, Ottleben I, Jacobsen H-J, Reinard T. (2005): Production of a Single-Chain Variable Fragment Antibody against Fumonisin B1.Journal of Agricultural and Food Chemistry 2005;53(4):899 - 904.
    DOI: 10.1021/jf048651s
  • Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Büttner, D., Caldana, C., Gaigalat, L., Goesmann, A., Kay, S., Kirchner, O., Lanz, C., Linke, B., McHardy, A. C., Meyer, F., Mittenhuber, G., Nies, D. H., Niesbach-Klösgen, U., Patschkowski, T., Rückert, C., Rupp, O., Schneiker, S., Schuster, S. C., Vorhölter, F., Weber, E., Pühler, A., Bonas, U., Bartels, D., and Kaiser, O. (2005): Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence.J. Bacteriol. 187, 7254-7266. More Info
  • Reinard T. (2004): Antikörper aus Pflanzen.Bioforum 2004;2004(12):38-39.
  • Frenzel A, Bergemann C, Köhl G, Reinard T. (2003): Novel purification system for 6xHis-tagged proteins by magnetic affinity separation.J Chromatogr B Analyt Technol Biomed Life Sci 2003;793(2):325-9.
  • Boch, J., Joardar, V., Gao, L., Robertson, T.L., Lim, M., and Kunkel, B.N. (2002): Identification of Pseudomonas syringae pv. tomato genes induced during infection of Arabidopsis thaliana. Mol. Microbiol. 44, 73-88. More Info
  • Hust M, Maiss E, Jacobsen HJ, Reinard T. (2002): The production of a genus-specific recombinant antibody using a recombinant potyvirus protease.J Virol Methods 2002;106(2):225.
  • Jackson, R.W., Mansfield, J.W., Ammouneh, H., Dutton, L.C., Wharton, B., Ortiz-Barredo, A., Arnold, D.L., Tsiamis, G., Sesma, A., Butcher, D., Boch, J., Kim, Y.J., Martin, G.B., Tegli, S., Murillo, J., and Vivian, A. (2002): Location and activity of members of a family of virPphA homologues in pathovars of Pseudomonas syringae and P. savastanoi. Mol. Plant Pathol. 3, 205-216. More Info
  • Willard J, Reinard T, Mohsen A, Vockley J. (2001): Cloning of genomic and cDNA for mouse isovaleryl-CoA dehydrogenase (IVD) and evolutionary comparison to other known IVDs [In Process Citation].Gene 2001;270(1-2):253-7.
  • Chen, Z., Kloek, A.P., Boch, J., Katagiri, F., and Kunkel, B.N. (2000): The Pseudomonas syringae avrRpt2 gene product promotes pathogen virulence from inside plant cells. Mol. Plant-Microbe Interact. 13, 1312-1321. More Info
  • Nennstiel, D., Boch, J., and Bonas, U. (2000): Bakterielle Phytopathogenese - Xanthomonas als Modellorganismus. Biospektrum 6, 23-26.
  • Reinard T, Janke V, Willard J, Buck F, Jacobsen HJ, Vockley J. (2000): Cloning of a gene for an acyl-CoA dehydrogenase from Pisum sativum L. and purification and characterization of its product as an isovaleryl-CoA dehydrogenase.J Biol Chem 2000;275(43):33738-43.
    DOI: DOI: 10.1074/jbc.M004178200
  • Ullrich, M.S., Schergaut, M., Boch, J., and Ullrich, B. (2000): Temperature-responsive genetic loci in the plant pathogen Pseudomonas syringae pv. glycinea.Microbiology 146, 2457-2468. More Info
  • Kappes, R.M., Kempf, B., Kneip, S., Boch, J., Gade, J., Meier-Wagner, J., and Bremer, E. (1999): Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.Mol. Microbiol. 32, 203-216. More Info
  • Nau-Wagner, G., Boch, J., Le Good, J.A., and Bremer, E. (1999): High-affinity transport of choline-O-sulfate and its use as a compatible solute in Bacillus subtilis. Appl. Environ. Microbiol. 65, 560-568. More Info
  • Boch, J., Verbsky, M.L., Robertson, T.L., Larkin, J.C., and Kunkel, B.N. (1998): Analysis of resistance gene-mediated defense responses in Arabidopsis thaliana plants carrying a mutation in CPR5.Mol. Plant-Microbe Interact. 11, 1196-1206. More Info
  • Hettwer, U., Jaeckel, F.R., Boch, J., Meyer, M., Rudolph, K., and Ullrich, M.S. (1998): Cloning, nucleotide sequence, and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. glycinea and P. syringae pv. phaseolicola. Appl. Environ. Microbiol. 64, 3180-3187. More Info
  • Reinard T, Achmus H, Walther A, Rescher U, Klaembt D, Jacobsen HJ. (1998): Assignment of the auxin binding abilities of ABP44 in gel.Plant and Cell Physiology 1998;39(8):874-878.
  • Boch, J., Nau-Wagner, G., Kneip, S., and Bremer, E. (1997): Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.Arch. Microbiol. 168, 282-289. More Info
  • Boch, J., Kempf, B., Schmid, R., and Bremer, E. (1996): Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.J. Bacteriol.. 178, 5121-5129. More Info
  • Reinard T, Jacobsen HJ. (1995): A soluble high affinity auxin-binding protein from pea apex.Journal of Plant Physiology 1995;147(1):132-138.
  • Sprunck S, Jacobsen HJ, Reinard T. (1995): Indole-3-lactic acid is a weak auxin analogue but not an anti-auxin.Journal of Plant Growth Regulation 1995;14(4):191-197.
  • Boch, J., Kempf, B., and Bremer, E. (1994): Osmoregulation in Bacillus subtilis: synthesis of the osmoprotectant glycine betaine from exogenously provided choline. J. Bacteriol. 176, 5364-5371. More Info
  • Reinard T, Jacobsen HJ. (1989): An inexpensive small volume equilibrium dialysis system for protein- ligand binding assays.Anal Biochem 1989;176(1):157-60.

Book Articles

  • Richter, A., Streubel, J., Boch, J. (2016): TAL effector DNA-binding principles and specificitiy.TALENs - Methods and Protocols, R. Kühn, W. Wurst, B. Wefers, eds., (New York: Springer), Methods in Molecular Biology, Vol. 1338, pp. 9-25. More Info
  • Richter, A., Streubel, J., and Boch, J. (2015): TAL effector DNA-binding principles and specificity.In R. Kühn, W. Wurst, B. Wefers (Eds.), TALENs: Methods and Protocols. Methods in Molecuar Biology. Vol. 1338, New York: Springer. pp. 9-25 More Info
  • Boch, J. (2009): The art of manipulation: bacterial type III effectors and their plant targets. In Plant pathogenic bacteria: genomics and molecular biology, R.W. Jackson, ed (Norfolk, UK: Caister Academic Press), pp. 241-271. More Info
  • Boch, J., and Bonas, U. (2001): Gram-negative plant pathogenic bacteria.In Emerging Bacterial Pathogens, I. Mühldorfer and K.P. Schäfer, eds (Basel: Karger), pp. 186-196. More Info

Other Publications

  • Reinard T. (2004): Farm im Turm in Biotechnologie 2020,Dechema Zukunftsforum
  • Reck B, Dietrich R, Bürk C, Lauer B, Reinard T. (2003): Innovative Nachweisverfahren für Mykotoxine.Transkript 2003;9(10).


  • Thomas Reinard (2018): Molekularbiologische Methoden 2.0UTB Verlag More Info
    ISBN: 9783825287429
  • Thomas Reinard (2010): Molekularbiologische MethodenISBN: 978-3-8252-8449-7 More Info