ResearchSections
Plant Molecular Biology

Plant Molecular Biology

Pflanzenmolekularbiologie

Plant molecular biology revolves around the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions:

Pflanzenmolekularbiologie

Plant molecular biology revolves around the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions:

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RESEARCH PROJECTS

  • Proteomic analysis of molecular mechanisms of resistance induction in plants

    We have analyzed the role of RAC/ROP proteins (r-related GTPases of plants) in the establishment of efficient symbiotic and pathogenic fungal infections in Medicago truncatula. RAC/ROP proteins are plant-specific small G proteins that function as molecular switches within elementary signal transduction pathways, including the regulation of reactive oxygen species (ROS) generation. Microbial infection is impaired by the activation of NADPH oxidase homologs of plants during early microbial infection. Proteomic profiling and expression studies suggest a fundamental role of RAC/ROP proteins in the establishment of efficient rhizobial and pathogenic infection.

  • Characterization of male sterile cytoplasms for hybrid breeding in cabbage (Brassica oleracea)

    Temperature dependent instability of the self-incompatibility system of Brassica makes a cytoplasmic male sterility system for hybrid breeding highly desirable. As no reliable CMS system was found in the genus Brassica, cytoplasms from Raphanus were transferred to Brassica by Japanese scientists. The most widely used cytoplasm is the „Ogura“ system which is now covered by several patents. Thus breeders are seeking alternative reliable cytoplasms.
    We have carried out a molecular genetic and biochemical analysis of the autoplasmic CMS “Tokumasu“ system and the alloplasmic CMS “tournefortii“. The male sterile plants of the latter system have a mitochondrial DNA which is recombined between Brassica tournefortii and Brassica napus. An open reading frome (orf 263) in the 3’ region of the mitochondrial gene for submit 6 of ATP-synthase codes a polypeptide of 263 amino acids. This orf 263 has been discussed by Landgren et al. (Plant Mol. Biol. 32, 879-890) as a candidate for causing pollen sterility. We found orf 263 not only in the sterile but also in the fertile line and sequenced both. Interestingly orf 263 is not transcribed in fertile or male sterile lines of our CMS “tournefortii“. On the other hand we have found differences in the transcription of other mitochondrial genes between male sterile and fertile lines.

  • Proteomic approach for the analysis of cytoplasmic male sterility in rape seed

    Proteome analysis is a powerful tool for the characterization of proteins involved in cytoplasmic male sterility. Our proteomic studies of CMS are based on the resolution of mitochondrial proteins by 2 D-gel electrophoresis and identification of the resolved proteins by mass spectrometry or protein sequencing. A comparative analysis of mitochondrial proteins from male sterile and fertile isogenic lines allows the direct identification of mitochondrial proteins which are quantitatively or qualitatively affected by the male sterile phenotype. Our investigations of the mitochondrial proteins from Brassica napus lines carrying genetic information from Brassica tournefortii in their mitochondria revealed characteristic features of the male sterile system “tournefortii“. An analysis of mitochondrial proteins from different tissues and developmental stages should provide more insights into the proteins affected by the male sterile phenotype.

CONTACT

Prof. Dr. rer. nat. Udo-Klaus Schmitz
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Herrenhäuser Straße 2
30419 Hannover
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Prof. Dr. rer. nat. Udo-Klaus Schmitz
Management
Address
Herrenhäuser Straße 2
30419 Hannover
Building
Room
Homepage of Prof. Dr. Udo-Klaus Schmitz