Besides the usual molecular biological methods for isolation and analysis of DNA and proteins we have experience in pretty new techniques, like EMP-PCR, POE-PCR, LAMP, and others.
We run an Äkta chromatography system and work with a surface plasmon resonance system (SPR, Autolab's Esprit system), which enables us to detect protein interaction to other proteins or small molecules with the highest sensitivity in real time.
The group uses 4 molecular biology labs, a tissue culture laboratory, 2 cultural chambers, and a greenhouse. Understanding the mode of action of the different methods is an important principle, as it can be seen in the textbook "Methods in Molecular Biology" by Thomas Reinard (sorry, only in German available).
Just now the following students spend their time in our lab:
Unfortunatelly, we are not able to offer any PhD thesis! Therefore our students leave us at the end of their master thesis.. Often, towards universities such as ETH Zurich, University of Vienna, Edinburgh, Aberdeen or Cambridge. Of course, some alumni stay in Hanover, change to Brunswick or to other German universities as Cologne, Freiburg, Giessen, Kiel and many more. Our alumni are also working in industry, e.g. at Roche AG, Lonza AG, DST, and others.
The textbook Molecular Biological Methods by Thomas Reinard bases on the lecture Molecular Biological Methods which is part of the Bachelor studies Life Science, Plantbiotechnology, Biology, and Biochemistry. More informations on the text book can be found here (German).
Our reviewed scientific publications are listed below. If you need the pdf of aone of our papers do not hesitate to contact me.
Ferro N, Bredow T, Jacobsen HJ, Reinard T. Route to Novel Auxin: Auxin Chemical Space toward Biological Correlation Carriers. Chem Rev. 2010 Jun 17
Ferro N, Bultinck, P, Gallegos, A, Jacobsen, H-J, Carbo-Dorca, R, Reinard, T. Unrevealed structural requirements for auxin-like molecules by
theoretical and experimental evidences. Phytochemistry, 2007, 68 (2) 237-250.
Ferro N, Gallegos, A, Bultinck, P, Jacobsen, H-J, Carbo-Dorca, R, Reinard, T. Coulomb and Overlap Self - Similarities: A comparative selectivity analysis of structure - function relationships for auxin - like molecules. J. Chem. Inf. Modeling (JCICS) 2006, 46(4):1751-1762
Ferro N, Tacoronte JE, Bultinck P, Reinard T, Montero LA. Structure–activity analysis on ecdysteroids: A structural and quantum chemical approach based on two biological systems. Journal of Molecular Structure: THEOCHEM 2006; 758, 2-3, 263-274 !SCIENCE DIRECT TOP25 Article! See here!
Lauer B, Ottleben I, Jacobsen H-J, Reinard T. Production of a Single-Chain Variable Fragment Antibody against Fumonisin B1. Journal of Agricultural and Food Chemistry 2005;53(4):899 - 904.
Reinard T. Antikörper aus Pflanzen. Bioforum 2004;2004(12):38-39.
Reinard T. Farm im Turm in Biotechnologie 2020, Dechema Zukunftsforum , (english pdf here, FTP)
Reck B, Dietrich R, Bürk C, Lauer B, Reinard T. Innovative Nachweisverfahren für Mykotoxine. Transkript 2003;9(10).
Frenzel A, Bergemann C, Köhl G, Reinard T. Novel purification system for 6xHis-tagged proteins by magnetic affinity separation. J Chromatogr B Analyt Technol Biomed Life Sci 2003;793(2):325-9.
Hust M, Maiss E, Jacobsen HJ, Reinard T. The production of a genus-specific recombinant antibody (scFv) using a recombinant potyvirus protease. J Virol Methods 2002;106(2):225.
Willard J, Reinard T, Mohsen A, Vockley J. Cloning of genomic and cDNA for mouse isovaleryl-CoA dehydrogenase (IVD) and evolutionary comparison to other known IVDs [In Process Citation]. Gene 2001;270(1-2):253-7.
Reinard T, Janke V, Willard J, Buck F, Jacobsen HJ, Vockley J. Cloning of a gene for an acyl-CoA dehydrogenase from Pisum sativum L. and purification and characterization of its product as an isovaleryl-CoA dehydrogenase. J Biol Chem 2000;275(43):33738-43.
Reinard T, Achmus H, Walther A, Rescher U, Klaembt D, Jacobsen HJ. Assignment of the auxin binding abilities of ABP44 in gel. Plant and Cell Physiology 1998;39(8):874-878.
Sprunck S, Jacobsen HJ, Reinard T. Indole-3-lactic acid is a weak auxin analogue but not an anti-auxin. Journal of Plant Growth Regulation 1995;14(4):191-197.
Reinard T, Jacobsen HJ. A soluble high affinity auxin-binding protein from pea apex. Journal of Plant Physiology 1995;147(1):132-138.
Reinard T, Jacobsen HJ. An inexpensive small volume equilibrium dialysis system for protein- ligand binding assays. Anal Biochem 1989;176(1):157-60.