Our team uses small plants, such as microalgae or duckweed, to produce pharmaceutically relevant proteins. Examples of such proteins are human interleukins and their receptors, antibodies of different formats (IgG, scFv, scFv-Fc) from various animal species, allergy-inducing proteins and some others.
The plants used by us all reveal extremely high growth rates and an easy cultivation. Besides the microalgae Chlamydomonas and Chlorella we increasingly use the saltwater diatom Phaeodactylum tricornutum. The duckweed Wolffia australiana is of particular interest due to its enormous potential as bioreactor. It is one of the smallest flowering plants in the world and both its genome and transcriptome was genome sequenced by our group recently. We use modern and highly efficient methods for our diverse molecular biological work. Our vector constructs are mainly based on the MoClo system, which is why, in addition to PCR-based cloning methods (EMP-PCR) and Gibson assembly, cut ligation is frequently used. All common transformation methods are available, both Agrobacteria-mediated and ballistic transformation as well as electroporation. In Wolffia we also use protoplasts, which we can produce in large quantities.
Finally, genome editing using CRISPR/Cas9 has also been performed in our group.
We are a key member of a variety of research collaborations. You can inform yourself about the most relevant ones on the following pages:
LACoP: Production of low allergy food plants such as peanuts or mustard
Summer Eczema: production of therapeutic antibodies for the treatment of summer eczema in horses
Besides the technical assistant Eva Plönnigs and the PhD student Stas Hans, the following students currently spend their days in our laboratory:
- Gerrich Behrendt (MSc Plant Biotechnology)
- Virginia Zahn (MSc plant biotechnology)
- Yahui Huang (MSc Molecular Microbiology)
- Matthias Schilder (BSc Biology)
- Ilka Kerren (BSc Plant Biotechnology)
- Florian Paulhttps://cmsv012b.t3luh.uni-hannover.de/11.html?&no_cache=1&tx_tkinstpersonen_pi1%5Balias%5D=FlorianP (BSc Plant Biotechnology)
- Linnea Tscheuschner (BSc Life Science)
Most students leave us after their Master's thesis, often to renowned universities such as ETH Zurich, the University of Vienna, Edinburgh, Aberdeen or Cambridge and of course Boston. Of course, some also stay in Hannover, move to Braunschweig or other German university cities such as Cologne, Freiburg, Giessen, Kiel and many more. Many of our alumni are now active in industry, in companies such as Yumab, Roche AG, Lonza AG, DST, KWS, Euroimmun, Eurofins and many more.
We do not have any funds to finance PhD students from abroad!
The textbook Molecular Biological Methods by Thomas Reinard bases on the lecture Molecular Biological Methods which is part of the Bachelor studies Life Science, Plantbiotechnology, Biology, and Biochemistry. More informations on the text book can be found here (German).
Our reviewed scientific publications are listed below. If you need the pdf of aone of our papers do not hesitate to contact me.
Ferro N, Bredow T, Jacobsen HJ, Reinard T. Route to Novel Auxin: Auxin Chemical Space toward Biological Correlation Carriers. Chem Rev. 2010 Jun 17
Ferro N, Bultinck, P, Gallegos, A, Jacobsen, H-J, Carbo-Dorca, R, Reinard, T. Unrevealed structural requirements for auxin-like molecules by
theoretical and experimental evidences. Phytochemistry, 2007, 68 (2) 237-250.
Ferro N, Gallegos, A, Bultinck, P, Jacobsen, H-J, Carbo-Dorca, R, Reinard, T. Coulomb and Overlap Self - Similarities: A comparative selectivity analysis of structure - function relationships for auxin - like molecules. J. Chem. Inf. Modeling (JCICS) 2006, 46(4):1751-1762
Ferro N, Tacoronte JE, Bultinck P, Reinard T, Montero LA. Structure–activity analysis on ecdysteroids: A structural and quantum chemical approach based on two biological systems. Journal of Molecular Structure: THEOCHEM 2006; 758, 2-3, 263-274 !SCIENCE DIRECT TOP25 Article! See here!
Lauer B, Ottleben I, Jacobsen H-J, Reinard T. Production of a Single-Chain Variable Fragment Antibody against Fumonisin B1. Journal of Agricultural and Food Chemistry 2005;53(4):899 - 904.
Reinard T. Antikörper aus Pflanzen. Bioforum 2004;2004(12):38-39.
Reinard T. Farm im Turm in Biotechnologie 2020, Dechema Zukunftsforum , (english pdf here, FTP)
Reck B, Dietrich R, Bürk C, Lauer B, Reinard T. Innovative Nachweisverfahren für Mykotoxine. Transkript 2003;9(10).
Frenzel A, Bergemann C, Köhl G, Reinard T. Novel purification system for 6xHis-tagged proteins by magnetic affinity separation. J Chromatogr B Analyt Technol Biomed Life Sci 2003;793(2):325-9.
Hust M, Maiss E, Jacobsen HJ, Reinard T. The production of a genus-specific recombinant antibody (scFv) using a recombinant potyvirus protease. J Virol Methods 2002;106(2):225.
Willard J, Reinard T, Mohsen A, Vockley J. Cloning of genomic and cDNA for mouse isovaleryl-CoA dehydrogenase (IVD) and evolutionary comparison to other known IVDs [In Process Citation]. Gene 2001;270(1-2):253-7.
Reinard T, Janke V, Willard J, Buck F, Jacobsen HJ, Vockley J. Cloning of a gene for an acyl-CoA dehydrogenase from Pisum sativum L. and purification and characterization of its product as an isovaleryl-CoA dehydrogenase. J Biol Chem 2000;275(43):33738-43.
Reinard T, Achmus H, Walther A, Rescher U, Klaembt D, Jacobsen HJ. Assignment of the auxin binding abilities of ABP44 in gel. Plant and Cell Physiology 1998;39(8):874-878.
Sprunck S, Jacobsen HJ, Reinard T. Indole-3-lactic acid is a weak auxin analogue but not an anti-auxin. Journal of Plant Growth Regulation 1995;14(4):191-197.
Reinard T, Jacobsen HJ. A soluble high affinity auxin-binding protein from pea apex. Journal of Plant Physiology 1995;147(1):132-138.
Reinard T, Jacobsen HJ. An inexpensive small volume equilibrium dialysis system for protein- ligand binding assays. Anal Biochem 1989;176(1):157-60.